THE 2-MINUTE RULE FOR POS88 SLOT

to review the effect of many constraints on mutational tolerance for the duration of selection, we selected E. coli dihydrofolate reductase (DHFR) like a model procedure. DHFR is an essential enzyme inside folate metabolism that minimizes dihydrofolate to tetrahydrofolate and it is needed for thymidine manufacturing. working with this exercise as T